Modifications and targeting of protein termini Part B



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Academic Press


Paru le : 2023-07-31



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Description
Modifications and Targeting of Protein Termini, Part B, Volume 686 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including In vitro production of N-degron fused proteins and its application, Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomics, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathway. Other chapters cover Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, Building libraries to dissect terminal degrons with fluorescent timers, Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS, Probing the effects of N-terminal acetylation on a-synuclein structure, aggregation and toxicity, Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE), and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Modifications and Targeting of Protein Termini
Pages
412 pages
Collection
n.c
Parution
2023-07-31
Marque
Academic Press
EAN papier
9780443221002
EAN PDF
9780443221019

Informations sur l'ebook
Nombre pages copiables
41
Nombre pages imprimables
41
Taille du fichier
21381 Ko
Prix
149,81 €
EAN EPUB SANS DRM
9780443221019

Prix
149,81 €

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