Proteomics Mass Spectrometry Methods

Sample Preparation, Protein Digestion, and Research Protocols

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Academic Press


Paru le : 2024-02-13



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Description
Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers. - Explores the most common questions new researchers have - Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses - Provides examples of sample preparation for a number of different materials, mammalian cells, and others
Pages
350 pages
Collection
n.c
Parution
2024-02-13
Marque
Academic Press
EAN papier
9780323903950
EAN PDF
9780323906524

Informations sur l'ebook
Nombre pages copiables
35
Nombre pages imprimables
35
Taille du fichier
3983 Ko
Prix
124,49 €
EAN EPUB SANS DRM
9780323906524

Prix
124,49 €

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